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luclite plus reporter gene assay  (Revvity)
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Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using <t>Luclite</t> plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.
Luclite Plus Reporter Gene Assay, supplied by Revvity, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/luclite+system/britelite+plus+Reporter+Gene+Assay+System/pmc09975258-317-12-17
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luclite plus reporter gene assay - by Bioz Stars, 2026-09
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luclite kit  (Packard Instruments)
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Packard Instruments luclite kit
Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using <t>Luclite</t> plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.
Luclite Kit, supplied by Packard Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/luclite+system/luclite+kit/pm29356860-54-7-9
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luclite kit - by Bioz Stars, 2026-09
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luclite cocktail  (Packard Instruments)
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Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using <t>Luclite</t> plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.
Luclite Cocktail, supplied by Packard Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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luclite cocktail - by Bioz Stars, 2026-09
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luclite assay kit  (Promega)
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Promega luclite assay kit
Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using <t>Luclite</t> plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.
Luclite Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/luclite+system/luclite+assay+kit/us09840556-89-22-25
Average 90 stars, based on 1 article reviews
luclite assay kit - by Bioz Stars, 2026-09
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luclite kit  (Promega)
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Promega luclite kit
Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using <t>Luclite</t> plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.
Luclite Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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luclite kit - by Bioz Stars, 2026-09
90/100 stars
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luclite luciferase reporter gene assay kit  (Packard Instruments)
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Packard Instruments luclite luciferase reporter gene assay kit
Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using <t>Luclite</t> plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.
Luclite Luciferase Reporter Gene Assay Kit, supplied by Packard Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/luclite+system/luclite+luciferase+reporter+gene+assay+kit/pm25022972-258-11-17
Average 90 stars, based on 1 article reviews
luclite luciferase reporter gene assay kit - by Bioz Stars, 2026-09
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luclite substrate  (Promega)
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Promega luclite substrate
Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using <t>Luclite</t> plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.
Luclite Substrate, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/luclite+system/luclite+substrate/pmc04743672-92-18-20
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luclite substrate - by Bioz Stars, 2026-09
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Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using Luclite plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.

Journal: Molecular & Cellular Proteomics : MCP

Article Title: HIV-1 Transcription Inhibitor 1E7-03 Decreases Nucleophosmin Phosphorylation

doi: 10.1016/j.mcpro.2022.100488

Figure Lengend Snippet: Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using Luclite plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.

Article Snippet: Cells were cultured 48 h post-transfection, and luciferase activity was analyzed using Luclite plus Reporter Gene Assay (PerkinElmer) measured by Glo-Max Microplate Multimode reader (Promega).

Techniques: Phospho-proteomics, Expressing, Transfection, Luciferase, Plasmid Preparation, Activity Assay, Reporter Gene Assay, Activation Assay, SDS Page, Control, Virus